| International tuition | Kč19,000 to Kč400,000 per year |
| Application fee | Not listed |
Studies in Czech at Masaryk are free, as at every Czech public university. English-taught degrees are charged per academic year, and the university sets the fee faculty by faculty in two currencies, so the headline range above covers the crown-priced faculties only. In Czech crowns: the Faculty of Medicine charges CZK 380,000 a year for General Medicine and CZK 400,000 for Dentistry; the Faculty of Arts CZK 76,000 for all bachelor's programmes and for the follow-up master's degrees in English Language and Literature and North-American Culture Studies, falling to CZK 38,000 for Medieval Latin Language and Literature and CZK 19,000 for Visual Cultures and Art History; the Faculty of Education CZK 68,000; and the Faculty of Economics and Administration CZK 100,000 for Economics and Public Policy and for Public Administration and CZK 120,000 for the rest. In euros: the Faculty of Science charges EUR 3,000 for most programmes, EUR 4,500 for Geography of Global Environmental Change and EUR 14,000 for Data Analytics; the Faculty of Informatics EUR 4,500; the Faculty of Pharmacy EUR 7,500; and the Faculty of Social Studies EUR 3,000 for studies starting in September 2026, rising to EUR 3,500 for a February 2027 start. Fees are paid per academic year.